a, By activating mdg4 RNAi from embryonic to adult stage, the survival rates were measured by raising flies on virus-containing food for 20 days. sh-white flies served as controls. All viral infection assays from this manuscript were performed with at least two biological replicates. Each replicate contains 20 males and 20 females. b, With mdg4 only silenced at pupal stage, the survival rates were measured by raising flies on virus-containing food for 20 days. c, With mdg4 only suppressed at adult stage, the survival rates were measured by raising flies on virus-containing food for 20 days. Flies used in panel a have one copy of the mdg4 reporter. Flies used in panels b and c only have endogenous copies of mdg4. Comparison of survival curves for all panels was completed using a Cox proportional-hazards model.