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. Author manuscript; available in PMC: 2022 Dec 28.
Published in final edited form as: Nat Genet. 2022 Nov 17;54(12):1933–1945. doi: 10.1038/s41588-022-01214-9

Fig. 5 |. mdg4 activation at pupal stage safeguards adult flies upon one-time viral infections.

Fig. 5 |

a, By activating mdg4 RNAi from embryonic to adult stage, the survival rates were measured after infecting adult files with DCV or FHV from single meal. Comparison of survival curves was completed using a Cox proportional-hazards model. b, By activating mdg4 RNAi from embryonic to adult stage, RT-PCR experiments were performed to monitor the amount of DCV in fly bodies after one-time infection. c, With mdg4 only silenced at pupal stage, RT-PCR experiments were performed to monitor the amount of DCV in adult flies after one-time infection. d, With mdg4 only silenced at adult stage, RT-PCR experiments were performed to monitor the amount of DCV in fly bodies after one-time infection. Three independent biological replicates were performed for panels b, c, and d.