Skip to main content
. Author manuscript; available in PMC: 2022 Dec 28.
Published in final edited form as: Nat Genet. 2022 Nov 17;54(12):1933–1945. doi: 10.1038/s41588-022-01214-9

Fig. 8 |. dSTING triggers Relish activation at the pupal stage for adult anti-viral responses.

Fig. 8 |

a, RT-qPCR to measure the RNAi silencing efficiency of dSTING based on the two-tailed t-test. Data are normalized to rp49 (RpL32) expression; the bars report mean ± standard deviation for three biological replicates. b, Immunostaining to detect the nuclear Relish-N signals in the posterior part of hindgut (top panel) and fat body cells (bottom panel) from sh-white and sh-dSTING early pupae. Silencing dSTING resulted in a significant decrease of the nuclear Relish-N in both hindgut and fat body cells. The flies were raised on germ-free condition. The data were collected from 2 biological replicates with 3 animals per replicate. Box plots report the minimum, maximum, median, and interquartile ranges of the data. A two-tailed t-test was used to compare the relative intensities of each group. c, With dSTING only silenced at pupal stage, the survival rates were measured by raising flies on virus-containing food for 20 days. Comparison of survival curves was completed using a Cox proportional-hazards model. d, With dSTING only silenced at pupal stage, RT-PCR experiments were performed to monitor the amount of DCV in adult flies after one-time infection. Three independent biological replicates were performed.