| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Urethane | Sigma-Aldrich | Cat# U2500; CAS:51-79-6 |
| Chlorprothixene hydrochloride | Sigma-Aldrich | Cat# C1671; CAS: 6469-93-8 |
| Isoflurane | Southern Anesthesia & Surgical | Cat# PIR001325-EA / 60307-120-25 |
| Bupivacaine hydrochloride (5 mg/mL) | Baxter Healthcare | Cat# 223238 |
| Pentobarbitone sodium (Lethabarb Euthanasia Injection) (325 mg/mL) | Virbac | Cat# 47815 Chemwatch: 3729556 |
| Sterile injectable saline | Alpha Medical Solutions | Cat# BBR3521340-P |
| Silicon oil (viscosity 500 cSt) | Sigma-Aldrich | Cat# 378380 CAS: 63148-62-9 |
| Paraformaldehyde | Sigma-Aldrich | Cat# 158127; CAS: 30525-89-4 |
| Biocytin | Sigma-Aldrich | Cat# B4261; CAS: 576-19-2 |
| Hydrogen peroxide (30%) | VWR | Cat# BDH7690-1; CAS: 7722-84-1 |
| Triton X-100 | Sigma-Aldrich | Cat# X100; CAS: 9036-19-5 |
| Bovine serum albumin (BSA) | VWR | Cat# 421501J; CAS: 9048-46-8 |
| Vectastain Elite ABC HRP-Kit | Vector Laboratories | Cat# PK-6100 |
| DAB substrate kit, peroxidase (HRP) | Vector Laboratories | Cat# PK-4100 |
| VectaMount Permanent Mounting Medium | Vector Laboratories | Cat# H-5000-60 |
| Tissue-Tek OCT compound | Sakura | Cat# 4583 |
| Sodium chloride | Sigma-Aldrich | Cat# S7653 CAS: 7647-14-5 |
| Potassium chloride | Sigma-Aldrich | Cat# P9333 CAS: 7447-40-7 |
| HEPES | Sigma-Aldrich | Cat# 54457 CAS: 7365-45-9 |
| Magnesium chloride hexahydrate | Sigma-Aldrich | Cat# M2670 CAS: 7791-18-6 |
| Calcium chloride | Sigma-Aldrich | Cat# C1016 CAS: 10043-52-4 |
| Potassium hydroxide | Sigma-Aldrich | Cat# 757551 CAS: 1310-58-3 |
| Potassium gluconate | Sigma-Aldrich | Cat# P1847 CAS: 299-27-4 |
| Adenosine 5′-triphosphate magnesium salt | Sigma-Aldrich | Cat# A9187 CAS: 74804-12-9 |
| Guanosine 5′-triphosphate sodium salt hydrate | Sigma-Aldrich | Cat# G8877 CAS: 36051-31-7 |
| Phosphocreatine disodium salt hydrate | Sigma-Aldrich | Cat# P7936 CAS: 19333-65-4 |
| Sodium phosphate dibasic dihydrate | Sigma-Aldrich | Cat# 71643 CAS: 10028-24-7 |
| Potassium phosphate monobasic | Sigma-Aldrich | Cat# P0662 CAS: 7778-77-0 |
| Hydrochloric acid | Sigma-Aldrich | Cat# 258148 CAS: 7647-01-0 |
| Ethanol | Sigma-Aldrich | Cat# 1.00986 CAS: 64-17-5 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J (male/female, 4–8 weeks old) | The Jackson Laboratory | Strain# 00064; RRID: IMSR_JAX:000664 |
| Software and algorithms | ||
| AxoGraph | AxoGraph | Version: AxoGraph 1.7.6 |
| MATLAB | MathWorks | Version: R2012b |
| Psychtoolbox-3 | N/A | Version: 3.0 |
| Other | ||
| Air table with Faraday cage | Sutter Instrument | Cat# AT-3036 |
| Multiclamp 700B patch-clamp amplifier | Axon Instruments, Molecular Devices | Cat# 2500-0157 |
| InstruTECH LIH 8+8 digitizer | HEKA | Cat# 895035 |
| Head stage | Axon Instruments, Molecular Devices | Cat# CV-7B |
| Ag/AgCl reference electrode with silver lead wire | Warner Instruments | Cat# 64-1314 (E242) |
| Motorized micromanipulator | Sutter instrument | Cat# MP-285 |
| Homeothermic blanket system with flexible probe | Harvard Apparatus | Cat# 14-516-143 / 507222F |
| Stereoscope mounted on a movable arm | Wild Heerbrugg | Cat# M5A |
| Light source | Volpi | Intralux 5000 |
| Microscope with 10× eyepiece and 40× objective | Zeiss | Axiovert S100 |
| Barometric pressure monitor | World Precision Instruments | Cat# PM015R |
| Luer Valve Assortment Kit | World Precision Instruments | Cat# 14011 |
| Laboratory tubing (various diameters) | Sigma-Aldrich | Cat# BR143352 Cat# BR143355 Cat# BR143356 |
| Disposable tuberculin syringe with slip tip (1 mL) | BD | Cat# 309659 / BD309659 |
| Syringe with slip tip (10 mL) | BD | Cat# 303134 / 75846-758 |
| MILLEX GV Syringe Filter (0.22 μm) | Sigma-Aldrich | Cat# SLGV013SL |
| Microloader tips, Eppendorf | VWR | Cat# 89009-310 |
| Flaming/Brown type micropipette puller | Sutter Instrument | Cat# P-97 |
| Borosilicate glass (filamented, fire polished) | Sutter Instrument | Cat# BF150-86-7.5HP |
| Flexible arm with magnetic base | RS | Cat# 614-1517 |
| LED diode (5 V, 5 mm) | RS | Cat# 860-9696 / L-7113GD-5V |
| Monitor | Dell | Cat# P221H |
| Constant voltage heating platform | Harvard Apparatus | Cat# K01340 |
| Single animal anesthesia machine with isoflurane vaporizer | E-Z Systems | Cat# EZ-108SA |
| Anesthesia induction chamber | E-Z Systems | Cat# EZ-178 |
| Demi-plus dental curing UV-light | Kerr | Cat# 910860-2 |
| Dental drill | Osada | Cat# Success 40 |
| Absorption triangles - unmounted | Fine Science Tools | Cat# 18105-03 |
| Scalpel blades No. 22 | Fisher Scientific | Cat# 22-079-697 |
| Dissecting scissors - L 4 1/2 in., Sharp, straight | Sigma-Aldrich | Cat# Z265977 |
| Surgical micro curette | Fine Science Tools | Cat# 10082-15 |
| Dumont #3 Forceps | Fine Science Tools | Cat# 11231-30 |
| Dumont #5/45 Forceps | Fine Science Tools | Cat# 11251-35 |
| Dumont #7 Forceps - Curved | Fine Science Tools | Cat# 11274-20 |
| BD Microlance 18G needle | VWR | Cat# 613-3833 |
| BD Microlance 25G needle | VWR | Cat# 613-0902 |
| BD Microlance 30G needle | VWR | Cat# 613-3942 |
| Weighing boat (46 × 46 × 8 mm) | VWR | Cat# 611-0093 |
| Filter paper (55 mm) | Cytiva Whatman | Cat# 10292221 |
| OptiBond Universal - Bottle Kit | Kerr Dental | Cat# 36517 |
| Kulzer Venus Flow composite | DC Dental | Cat# 504-66014561 |
| Syringe with slip tip (50 mL) | BD | Cat# 309654 / BD309654 |
| Vaseline (white petrolatum) | VWR | Cat# 470301-948 |
| Sterile cotton swabs | VWR | Cat# HERE1030529 |
| Surgical scissors - serrated | Fine Science Tools | Cat# 14007-14 |
| Iris scissors (Extra Fine Bonn Scissors) | Fine Science Tools | Cat# 14083-08 |
| Graefe forceps - Curved 1.2 × 0.9 mm | Fine Science Tools | Cat# 11652-10 |
| Spatula/spoon combination | Fine Science Tools | Cat# 10090-17 |
| Hemostat - straight | Fine Science Tools | Cat# 13006-12 |
| Castroviejo scissors - curved | Fine Science Tools | Cat# 15017-10 |
| Stainless steel drying tray | Sigma-Aldrich | Cat# Z155977-1EA |
| Dissecting board | Fisher Scientific | Cat# 09-002-24A |
| Butterfly needle 25G | VWR | Cat# BRAU4056502-01 |
| Flacon tube (50 mL) | VWR | Cat# 734-0448 |
| Flacon tube (15 mL) | VWR | Cat# 734-0451 |
| Cryostat CM1950 | Leica | Cat# 14 0477 8U101 |
| Razor blade | VWR | Cat# 55411-050 |
| Cryostat cutting blade | Sakura | Cat# 4689 |
| 12-well plate | VWR | Cat# 470332-698 |
| Orbital shaker | Fisher Scientific | Cat# 10359009 |
| Fine brush | Fisher Scientific | Cat# NC0344756 |
| Tissue-Tek Cryomold | Sakura | Cat# 4566 |
| Petri dish | VWR | Cat# 391-0657 |
| Pasteur pipette | VWR | Cat# ELKA127-P503-000 |
| Microscope slides | VWR | Cat# 631-1553 |
| Coverslips | VWR | Cat# 631-0147 |