Artificial cerebrospinal fluid (aCSF)5
| Reagent | Final concentration (mM) | Amount |
|---|---|---|
| NaCl | 135 | 3.945 g |
| KCl | 5.4 | 0.201 g |
| HEPES | 5 | 0.596 g |
| MgCl2.6H2O | 1 | 0.102 g |
| CaCl2 | 1.8 | 0.1 g |
| Millipore water | N/A | Add to 500 mL |
Can be stored in sealed container at 4°C for two weeks.