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Intracellular solution (ICS)
| Reagent | Final concentration (mM) | Amount |
|---|---|---|
| KCl | 10 | 0.037 g |
| K-gluconate | 130 | 1.522 g |
| HEPES | 10 | 0.119 g |
| Mg-ATP | 4 | 0.101 g |
| Na2-GTP | 0.3 | 0.008 g |
| Na2-phosphocreatine | 15 | 0.191 g |
| Millipore water | N/A | Add to 50 mL |
Aliquots can be stored at −20°C for several months.
