10× phosphate-buffered saline (PBS)
| Reagent | Final concentration (mM) | Amount |
|---|---|---|
| NaCl | 1,370 | 80 g |
| KCl | 27 | 2 g |
| Na2HPO4.2H2O | 100 | 14.4 g |
| KH2PO4 | 18 | 2.4 g |
| Millipore water | N/A | Add to 1 L |
Can be stored in sealed container at 4°C for several weeks.
10× phosphate-buffered saline (PBS)
| Reagent | Final concentration (mM) | Amount |
|---|---|---|
| NaCl | 1,370 | 80 g |
| KCl | 27 | 2 g |
| Na2HPO4.2H2O | 100 | 14.4 g |
| KH2PO4 | 18 | 2.4 g |
| Millipore water | N/A | Add to 1 L |
Can be stored in sealed container at 4°C for several weeks.