Gelatin/Fibronectin solution (For 5 × 35 mm glass-bottom dish)

Reagent	Final concentration	Amount
2% Gelatin solution	0.1%	0.5 mL
Human Fibronectin (1 mg/mL)	20 μg/mL	0.2 mL
MycoZap™ (500×)	1×	0.02 mL
PBS	N/A	9.28 mL
Total	N/A	10 mL