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Plasmid solution for transfection per 35 mm glass-bottom dish
| Reagent | Final concentration | Amount |
|---|---|---|
| Plasmid (GFP-tagged GJA1-20k) | 0.5 μg/dish | N/A |
| Plasmid (LifeAct-mCherry) | 1.0 μg/dish | N/A |
| FuGene HD | 1:3 ratio (total plasmids μg : FuGene μL) | 4.5 μL (for 1.5 μL of total plasmids) |
| OptiMEM | N/A | Bring up volume to 125 μL |
| Total | N/A | 125 μL per dish |
