REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Dulbecco’s Modified Eagle Medium (DMEM)	Thermo Fisher Scientific	Cat# 11965-092 Cat# 21063-029
Fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat# 26140-079
MEM non-essential amino acids	Thermo Fisher Scientific	Cat# 11140-050
Sodium pyruvate	Thermo Fisher Scientific	Cat# 11360-070
MycoZap™ Plus-CL	Lonza	Cat# VZA-2012
Dulbecco’s phosphate buffered saline (PBS)	Thermo Fisher Scientific	Cat# 14190-144
Opti-MEMTM I Reduced Serum Medium	Thermo Fisher Scientific	Cat# 31985-062
FuGene HD	Promega	Cat# E2312
Lipofectamine RNAiMAX	Thermo Fisher Scientific	Cat# 13778150
Mitotracker™ Deep Red FM	Thermo Fisher Scientific	Cat# m22426
Gelatin solution	Sigma-Aldrich	Cat# G1393
Human fibronectin	Corning	Cat# 356008
Experimental models: Cell lines
HEK293FT cell line	Thermo Fisher Scientific	Cat# R70007
Recombinant DNA
Plasmid: GFP-tagged GJA1-20k	N/A	Fu et al.16
Plasmid: LifeAct-mCherry	Addgene	Cat# 40908
siRNA for Drp1 knock-down	Thermo Fisher Scientific	ID: 19561
siRNA control (stealth RNA)	Thermo Fisher Scientific	Cat# 12935300
Software and algorithms
NIS-Elements	Nikon Instruments Inc.	https://www.microscope.healthcare.nikon.com/products/software/nis-elements
ImageJ Fiji	Johannes Schindelin, Ignacio Arganda-Carreras, Albert Cardona, Mark Longair, Benjamin Schmid, and others	https://imagej.net/software/fiji/downloads
GraphPad Prism 9	GraphPad	https://www.graphpad.com
Other
35 mm glass-bottom culture dish	MatTek Corporation	Cat# P35G-1.0-14-c
Water bath	Precision	Model# 2864
Microscope	Nikon	Eclipse Ti2 (Inverted)
Microscope incubator for gas and temperature control	Okolab	https://www.oko-lab.com/
Objective	Nikon	100×/1.49 Apo TIRF
Spinning disk confocal unit with 486, 561, and 647 nm diode-pumped solid-state lasers (DPSSL)	Yokogawa	CSU-W1 SoRa
CMOS Disk camera	Photometrics	Prime BSI