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Cocultivation experiment using recombinant urease B subunit. MKN 45 cells were cocultivated in triplicate with recombinant urease B subunit in the presence of urea or H. pylori, as indicated. All cultures were treated with TNF-α and IFN-γ. Eight hours after incubation, the percent apoptosis and the concentration of ammonia were determined. The bars represent SDs.
