FIG. 3.

Relative quantities of Rgg-regulated gene transcripts assessed by TaqMan assays. cDNA detected from stationary-phase cultures of NZ131 wild-type (wt), speB, and rgg strains was quantified for mf, the DNA entry nuclease gene (orf226), orf953, the autolysin gene (orf1669), and clpB (orf204). The cDNA values were normalized to the quantity of gyrA cDNA in each sample. The experiments were repeated using at least two independently isolated RNA preparations, and representative results are shown.