TABLE 3.

Stationary-phase supernatant proteins identified by peptide mass fingerprinting

Designation	Name	Accession no.a	Mr/pIb	No. of peptides (coverage)c	Δppmd
SpeB-12	Streptokinase	M19347	50,084/5.54	8 (13)	2.8 ± 6.0
SpeB-15	Glyceraldehyde-3-phosphate dehydrogenase/plasmin receptor	M95569	35,958/5.34	8 (30)	2.9 ± 4.5
SpeB-19	6-Phosphofructokinase	RST00803	35,748/5.33	7 (23)	0.8 ± 2.6
SpeB-20	Immunogenic secreted protein	RST00809, U31811	53,292/5.5	5 (10)	8.2 ± 7.2
SpeB-22	Streptokinase	M19347	50,084/5.54	5 (14)	1.3 ± 3.6
SpeB-25	Streptokinase	M19347	50,084/5.54	5 (18)	3.3 ± 9.6
SpeB-26	DNA entry nuclease	RST00226	30,398/4.93	4 (16)	0.3 ± 5.8
SpeB-27	CAMP factor	RST0342, AF079502	28,480/6.25	4 (15)	6.9 ± 1.8
SpeB-28	ClpB	RST00204	63,349/5.41	5 (9)	10.4 ± 17.8
SpeB-33	CAMP factor	RST0342, AF079502	28,480/6.25	5 (26)	2.8 ± 2.7
SpeB-34	CAMP factor	RST0342, AF079502	28,480/6.25	6 (29)	0.1 ± 4.9
SpeB-35	CAMP factor	RST0342, AF079502	28,480/6.25	6 (29)	0.1 ± 4.9
SpeB-37	Enolase	RST00465	37,330/4.83	6 (23)	7.1 ± 1.2
SpeB-38	Autolysin	RST01669	23,020/4.66	4 (15)	11.7 ± 2.7
SpeB-40	Lysozyme	RST01324	49,729/5.04	5 (15)	1.5 ± 16.5
SpeB-43	Mitogenic factor	D13428	30,061/9.24	7 (37)	7.6 ± 1.8
SpeB-46	Ribosome recycling factor	RST00322	20,572/5.68	8 (45)	11.7 ± 4.7
Rgg-5	Streptokinase	M19347	50,084/5.54	8 (23)	1.7 ± 3.2
Rgg-8	Streptokinase	M19347	50,084/5.54	7 (17)	5.4 ± 6.7
Rgg-17	DNA entry nuclease	RST00226	30,398/4.93	8 (23)	4.2 ± 3.5
Rgg-18	Immunogenic secreted protein	RST00809, U31811	53,292/5.50	5 (37)	4.7 ± 5.5
Rgg-19	DNA entry nuclease	RST00226	30,398/4.93	6 (27)	6.2 ± 3.1
Rgg-20	Unknown	RST00953	32,053/5.79	4 (22)	2.6 ± 6.2
Rgg-21	Streptokinase	M19347	50,084/5.54	9 (20)	1.7 ± 1.7
Rgg-23	CAMP factor	RST0342, AF079502	28,480/6.25	10 (38)	6.0 ± 2.6
Rgg-30	Immunogenic secreted protein	RST00809, U31811	53,292/5.50	4 (11)	15.2 ± 13.4
Rgg-32	Streptokinase	M19347	50,084/5.54	7 (18)	18.7 ± 6.1
Rgg-36	DNA entry nuclease	RST00226	30,398/4.93	5 (24)	1.0 ± 4.1
Rgg-37	Streptokinase	M19347	50,084/5.54	8 (13)	1.7 ± 7.3
Rgg-38	Phosphoglycerate kinase	RST00513	42,130/4.82	5 (17)	4.9 ± 11.8
Rgg-39	Mitogenic factor	D13428	30,061/9.24	5 (28)	18.3 ± 9.1
Rgg-40	Mitogenic factor	D13428	30,061/9.24	8 (37)	9.1 ± 5.0
Rgg-42	Mitogenic factor	D13428	30,061/9.24	9 (45)	3.1 ± 15.9
Rgg-43	Mitogenic factor	D13428	30,061/9.24	4 (19)	11.4 ± 10.6
Rgg-44	DNA entry nuclease	RST00226	30,398/4.93	6 (27)	11.2 ± 1.9
Rgg-45	Mitogenic factor	D13428	30,061/9.24	5 (26)	18.0 ± 7.6
Rgg-47	DNA entry nuclease	RST00226	30,398/4.93	6 (14)	11.2 ± 1.9

^aNCBI accession number or WIT2 RST designation. 

^bInferred from the nucleotide sequence. 

^cNumber of tryptic peptides of the identified protein detected by MALDI-TOF MS (percentage of the protein covered with detected peptides). 

^dMean difference ± standard error between masses detected by MALDI-TOF MS and calculated masses of the tryptic peptides.