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Galectin-3-induced production of oxygen radicals in neutrophil cytoplasts. Cytoplasts resuspended to a concentration of 106/ml were stimulated with galectin-3 (20 μg/ml). The extracellular release of superoxide anion was measured by isoluminol-amplified CL in the presence of HRP, and the CL response is given as 106 cpm (Mcpm). Kinetics of a representative experiment are shown. The mean peak value (±SD) of three experiments was 20.4 ± 7.2 Mcpm.
