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Extracellular release of gelatinase and vitamin B12-binding protein from LPS-primed and unprimed neutrophils. Neutrophils were preincubated for 30 min in presence or absence of LPS (10 μg/ml) at 4 or 37°C. Bars represent release into the medium of gelatinase (marker for the gelatinase and specific granules) and vitamin B12-binding protein (marker for the specific granules), as a percentage (mean + SD, n = 5) of the total amount in control cells.
