FIGURE 3.

Effect of 6‐hour (6h) and 16‐hour (16h) fasts with different cage change methods on intraperitoneal glucose tolerance test (IPGTT) outcome in (A) males, (B) females in proestrous‐oestrous (P‐E) and (C) females in metoestrous‐dioestrous (M‐D). (D) Area under the curve (AUC)₁₂₀ for graphs A‐C. * and # represent a significant difference compared to females in P‐E and M‐D, respectively. ~, + and = represent a significant difference compared to 6 hours with whole cage change (WCC), 6 hours with bedding retention cage change (BRCC) and 6 hours with no cage change (NCC), respectively (P < 0.05, two‐way repeated‐measures ANOVA with Holm‐Sidak post hoc tests). Data are mean ± SEM (n = 7 for males, females in P‐E and females in M‐D)