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Effect of purified pneumolysin on HBMEC detachment 24 h after addition of the protein in comparison to results for pneumococci (D39; 107 CFU/ml). Values are means of duplicate results; error bars are standard deviations. ∗ and ∗∗, P values of <0.01 and <0.001 with respect to controls. The hemolytic titers of the culture medium after supplementation with 30 μg of pneumolysin/ml and 107 CFU of pneumococci/ml were comparable.
