TABLE 2.
Semi‐quantitative evaluation of histochemical staining
| Protein | Total bone ECM | Cortical bone | Trabecular bone | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Healthy bone | OP bone | p‐value | Healthy bone | OP bone | p‐value | Healthy bone | OP bone | p‐value | |
| COL1A1 | 91.82% ± 4.80 | 81.80% ± 10.27 | 0.0002 | 91.50% ± 3.94 | 84.33% ± 6.11 | 0.01 | 92.14% ± 6.19 | 79.57% ± 13.08 | 0.01 |
| COL1A2 | 93.23% ± 1.89 | 88.20% ± 4.17 | 0.0001 | 93.30% ± 2.12 | 89.70% ± 2.65 | 0.0016 | 93.16% ± 1.75 | 86.97% ± 4.86 | 0.0004 |
| DCN | 19.11% ± 6.67 | 29.47% ± 13.19 | 0.002 | 17.55% ± 11.72 | 30.89% ± 13.5 | 0.006 | 20.80% ± 5.58 | 28.04% ± 13.31 | 0.235 |
| OCN | 14.95% ± 7.86 | 28.01% ± 12.46 | 0.0003 | 16.17% ± 7.50 | 33.38% ± 14.61 | 0.01 | 13.87% ± 8.45 | 25.57% ± 10.31 | 0.009 |
| OPN | 22.77% ± 5.95 | 15.94% ± 6.09 | 0.0002 | 22.17% ± 5.67 | 14.19% ± 3.32 | 0.002 | 23.75% ± 6.30 | 17.88% ± 7.89 | 0.02 |
| BSP‐2 | 11.86% ± 7.93 | 10.42% ± 6.58 | 0.678 | 14.08% ± 9.07 | 11.51% ± 7.73 | 0.651 | 9.65% ± 3.22 | 9.32% ± 5.31 | 0.862 |
| ON | 11.12% ± 7.11 | 10.57% ± 4.18 | 0.620 | 14.20% ± 8.16 | 11.51% ± 3.80 | 0.798 | 7.76% ± 5.44 | 9.45% ± 4.51 | 0.449 |
| TGF‐β | 2.99% ± 1.57 | 4.71% ± 2.64 | 0.116 | 3.68% ± 1.65 | 4.97% ± 2.05 | 0.699 | 2.25% ± 1.16 | 4.83% ± 3.05 | 0.222 |
Abbreviations: COL1A1 and COL1A2, Type I collagen; DCN, Decorin; OCN, Osteocalcin; OPN, Osteopontin; BSP‐2, bone sialoprotein‐2; ON, Osteonectin; TGF‐β, transforming growth factor β.