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Surface F and G expression on HEp-2 cells infected with RSV G-mutant vaccines by flow cytometry. Gating was performed on live cells and singlets, and infected cells were gated based on mKate2 expression. Reactivity of RSV F (motavizumab) and G (3D3) monoclonal antibodies was measured using immunofluorescence. Abbreviations: FSC, forward scatter; RSV, respiratory syncytial virus; SSC, side scatter.
