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. 2022 Sep 27;2(9):e536. doi: 10.1002/cpz1.536

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© 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Lifting organotypic cultures to an air liquid interface. (A and B) Carefully break the connection between the collagen plug and the side of the well by pressing a sterile lab spatula up and down around the edge. Do not run the spatula around the edge once pressed down. This can cause the cells on the plug to pull from the plug itself. (C and D) Using the sterile lab spatula, scoop underneath the collagen plug and place the collagen plug onto the metal grid with E‐medium underneath the collagen plug.