Figure 5.

Whole mount staining of organotypic cultures. (A) Example images showing whole mount stained organotypic cultures stained with DAPI and S100B to label melanocytes. Images were collected using a Nikon A1R confocal laser scanning microscope equipped with GaAsP detectors and 20× Plan‐Apochromat objective with an NA of 0.75. NIS‐Elements were used to generate 3D images of z stacks using the alpha‐blend method with z‐depth color coding. Lookup table represents z depth based on voxel color, scale bar = 50 μm. (B) Example images of whole mount samples taken with a brightfield microscope. These samples were not stained allowing visualization of the pigmented melanocytes and any pigment released into the culture. Scale bar = 50 μm.