Table 1. Primer/probe sets for detection of Bourbon virus RNA in New York, NY, USA*.
| Name | Gene target | Sequence, 5′ → 3′ |
|---|---|---|
| BRBV F† | Polymerase subunit, PB1 | AACCGGCCAATAGGG |
| BRBV R | Polymerase subunit, PB1 | TGCCAGTTGGGTAGC |
| BRBV PROBE_5Cy5 | /5Cy5/ATGGAGCTG/TAO/CTTTCACTACC/3IAbRQSp/ | |
| Bourbon_virus_F1‡ | Polymerase subunit, PB1 | ATTGCTACTCCGTCCATGTTAGTAAG |
| Bourbon_virus_R1 | Polymerase subunit, PB1 | CCAGAACTTGGTAGACATTCCAATAAG |
| Bourbon_virus_P1_HEX probe | /5HEX/CCCTTGCTG/ZEN/CATCTTCCACCACTTTCACAA/3IABkFQ/ |
*BRBV, Bourbon virus; F, forward; P, probe; PB1, polymerase basic 1; R, reverse. †Primer/probe set developed at Wadsworth Center, New York State Department of Health, based on Bourbon virus (St. Louis strain) (GenBank accession no. MK453528). ‡Primer/probe set developed at TickReport (https://www.tickreport) based on Bourbon virus (original strain) (GenBank accession no. KU708254)