Assessment of 3 tissue Iso‐Seq (brain, gonad, heart) Sturnus vulgaris transcriptome. (a) busco (aves) rating summaries for S. vulgaris short read liver transcriptome, the high‐quality Iso‐Seq S. vulgaris transcript produced though the Iso‐Seq version 3.3 pipeline, the final S. vulgaris transcriptome produced by tama collapse pipeline, and combined high‐quality Iso‐Seq and short read liver transcripts, alongside two other avian Iso‐Seq transcriptomes (Anas platyrhynchos using pectoralis, heart, uterus, ovary, testis, hypothalamus, pituitary and 13 day‐old embryo tissue (Yin et al., 2019), and Calypte anna using liver tissue (Workman et al., 2018)). (b) Breakdown of major gene ontology (GO) terms in the sequenced Iso‐Seq reads, with cellular component (red) molecular function (blue) and biological process (green)