Schematic representation of the promoter and antigen regions of shuttle vectors pEL17CRM197, pEL71CRM197, and pEL73CRM197. All vectors contain E. coli and mycobacterial origins of replication, a kanamycin resistance gene (Kanr), pBlaF*, and CRM197. pEL71CRM197 also has the β-lactamase signal sequence (ss Blam), while pEL73CRM197 has the complete β-lactamase (Blam) sequence fused to the CRM197 sequence.