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. 2022 Jul 10;20(10):2350–2365. doi: 10.1111/jth.15794

FIGURE 3.

FIGURE 3

Breast cancer cell‐induced angiogenesis is inhibited by LMWH. MDA‐MB‐231 and MCF‐7 cells were cultured alone or with platelets (plts), in the presence and absence of anti‐VEGF‐A antibody bevacizumab (100 ng/ml) or LMWH (100 IU/ml). Tumor supernatant was then used for a HUVEC angiogenesis assay using growth factor reduced matrigel. HUVECs were cultured with the tumor supernatant from (A, B) MDA‐MB‐231 or (C, D) MCF‐7 and representative images of capillary tube formation generated were taken with Zeiss Axiovert microscope. Quantification of number of EC nodes formed following exposure to tumor supernatant from (B) MDA‐MB‐231 and (D) MCF‐7. Data are presented as the mean ± SEM of at least three independent experiments. Data were analyzed with anova followed by Bonferroni t‐ test (*p < .05, **p < .005, ***p < .001). Scale bar 100 μm.