FIGURE 6.

LMWH attenuates breast tumor transendothelial migration. Transendothelial migration of (A) MDA‐MB‐231 and (B) MCF‐7 breast tumor cells was assessed using fluorescently labeled tumor cells seeded on top of HUVEC confluent monolayer. Various stimuli were used to alter endothelial integrity including VEGF‐A, supernatant from breast tumor cell alone of following their co‐culture with platelets. After 24 h, tumor cells that had migrated into bottom chamber were lysed to measure the relative fluorescence units (RFU). Data are presented as the mean ± SEM of at least three independent experiments. Data were analyzed with anova followed by Bonferroni t‐test and p values < .05 were considered to be significant (*p < .05, **p < .005, ***p < .001).