FIGURE 2.

Expression of three cluster 4 proximal genes in X. laevis hind limb buds. (A‐I) Whole mount in situ hybridization (purple staining) of stages 50 to 52 hind limb buds as viewed from the dorsal side, posterior uppermost. (A‐C) white arrows indicate proximal spot of mesenchymal gata5.L at stage 50 (A), 51 (B), and 52 (C). (D‐F) irx5.L in the proximal mesenchyme at stage 50 (D), 51 (E), and 52 (F). Black stellate cells are melanophores in nonalbino tadpoles (D‐F), expression in stage 52 is anterior as well as proximal. (G‐I) Expression of tnn.L is very strong along the boundary with the body wall, and is epithelial. Stage 50 limb bud dissected to enable visualization of limb bud, as there is strong expression in the ventral fin as well as the limb bud (G), stage 51 (H), and 52 (I). Scale bar in (I) is 250 μM and applies to all panels. (J), Normalized read counts of gata5.L, irx5.L and tnn.L in the three sections of the stage 51 limb bud transcriptome. Red and black dots indicate biological replicate data, where only red can be seen it overlaps completely with black