| Gene name | Forward primer 5′–3′ | Reverse primer 5′–3′ |
|---|---|---|
| ENG | AGCCCCACAAGTCTTGCAG | GCTAGTGGTATATGTCACCTCGC |
| COL1A1 | CCCCTGGAAAGAATGGAGATG | TCCAAACCACTGAAACCTCTG |
| OCN | GGCGCTACCTGTATCAATGG | TCAGCCAACTCGTCACAGTC |
| ALPL | ACGTACAACACCAATGCCC | GGTCACAATGCCCACAGATT |
| RUNX2 | CTGTGGTTACTGTCATGGCG | AGGTAGCTACTTGGGGAGGA |
| GAPDH | GGTATCGTGGAAGGACTCATGAC | ATGCCAGTGAGCTTCCCGTTCAG |
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