Rate of stachylysin hemolysis of sheep RBCs. Approximately 0.2 μg of purified stachylysin in 10 μl of sterile, deioinzed water was spotted onto an SBA plate. The plate was incubated at 37°C and photographed at intervals of 0.25, 0.5, 1.0, 6.0, 24, and 48 h during the incubation.