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. 2022 Dec 14;16:1074304. doi: 10.3389/fncel.2022.1074304

FIGURE 1.

FIGURE 1

Endogenous tagging of unc-13 in Drosophila melanogaster. (A,B) Genetic map of unc-13 in Drosophila with the five annotated transcripts A, C, F, G, and D (A) and the two annotated transcripts B and E (B). Black boxes indicate exons, gray boxes introns and green boxes mark the insertion site of the EGFP-FlAsH-StrepII-TEV-3xFlag (GFSTF)-tag located in a coding intron. Lower panels show amino acids of the Unc-13A and Unc-13B fly protein illustrating the specific elements the GFSTF-tag consists of. (C) Schematic illustration of Unc-13A and Unc-13B domains: CaM- (Calmodulin), C1-, C2 B-, C2 C-, and MUN-domain and the PxxP-motif. Green box marks GFSTF-tag, magenta bars indicate epitopes of Unc-13A and Unc-13B antibodies (Böhme et al., 2016) and blue bars of N- and C-term antibodies, respectively (Reddy-Alla et al., 2017).