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. 2022 Dec 14;18(12):e1010549. doi: 10.1371/journal.pgen.1010549

Fig 7. Distribution of ergosterol is altered in the drs2 mutant.

Fig 7

A) The ergosterol reporters Filipin and D4H localize similarly at the apex of the filament in wild-type cells. Top panel: Wild-type cells (PY4861) were induced serum prior to staining with filipin, as described [58]. Images were taken with a wide-field fluorescence microscope. Bottom panel: Wild-type cells expressing mScarlet-D4H together with Trq-CtRac1 (PY6237) were induced with serum as in Fig 1B and images were taken with a spinning disk confocal microscope and central z-section, as well as merge images, are shown. B) Ergosterol distribution is altered in the drs2 mutant. Images of drs2/drs2 cells (drs2, PY6083) and drs2/drs2 + pDRS2DRS2 cells (drs2 + DRS2, PY6218) expressing mScarlet-D4H were taken as in Fig 6A bottom panel.