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Protection of mice immunized s.c. with FBP54 against lethal infection with heterologous S. pyogenes strains. Groups of mice (n = 15) were injected intraperitoneally with strain SSI-9 (M1; 107 CFU) or strain S42 (M12; 3 × 107 CFU) on day 51 after the first immunization. Mortality was monitored every day. χ2 analysis was employed to analyze the significance of differences between the means of FBP54-immunized and nonimmunized mice on day 8. ∗, P < 0.05; ∗∗, P < 0.001.
