. 2001 Feb;69(2):924–930. doi: 10.1128/IAI.69.2.924-930.2001

TABLE 1.

Experimental protocol for immunization with FBP54

Group^a	Vaccine/adjuvant used	Procedure^b on postimmunization day:
Group^a	Vaccine/adjuvant used	0	14	28	42	51
I	FBP54/FCA	s.c.	s.c.	s.c.	s.c.	Challenge
II	FBP54/CT	i.n.	i.n.	i.n.	i.n.	Challenge
III	FBP54/CT	p.o.	p.o.	p.o.	p.o.	Challenge
IV	CT	p.o.	p.o.	p.o.	p.o.	Challenge
V	None					Challenge

Each group consisted of five female six-week-old BALB/c mice.

Each vaccine was injected biweekly for a total of four inoculations. Blood and saliva samples were collected on days 0, 14, 28, and 49. Mice were challenged with S. pyogenes on day 51. s.c., s.c. injection with an emulsion of FBP54 and FCA; i.n., intranasal immunization with 10 μg of FBP54 and 10 μg of CT; p.o., peroral inoculation with 20 μg of FBP54 and 10 μg of CT or with 10 μg of CT alone.