Figure 4.

A. IHC images showing reactive astrocytes and microglia of healthy tissue and 5 days post stroke. B. Schematic of experimental timeline where arrow signifies stroke day, + signifies injection day (MAP only), and x signifies sacrifice and analysis time point. Sham is stroke only with no injection. C. IHC images of sham stroke tissue showing reactive astrocytes and reactive microglia at 1-, 2-, 4-weeks post stroke through GFAP and Iba1 staining. D. IHC images of MAP gel treated stroke tissue showing reactive astrocytes and reactive microglia at 1-, 2-, 4-weeks post stroke through GFAP and Iba-1 staining. E. Quantification of scar thickness over time through GFAP staining comparing sham and HA-MAP treated brains. F. Quantification of percent area of reactive astrocytes in the peri-infarct through GFAP staining comparing sham and HA-MAP treated brains. G. Quantification of astrocyte infiltration into infarct through GFAP staining comparing sham and HA-MAP treated brains. H. Quantification of percent area of reactive microglia in peri-infarct area through Iba-1 staining comparing sham and HA-MAP treated brains. I. Quantification of percent area of reactive microglia in infarct through Iba-1 staining comparing sham and HA-MAP treated brains. All scale bars = 100µm. All measurements were done on FIJI. Statistical analysis was done in GraphPad Prism using two-way ANOVA analysis of variance followed by a multiple comparisons test (Sidak) comparing between condition at each timepoint. The dots in each plot indicate the number of animals (n = 5 biological replicates) that were analyzed per-group and were considered independent experiments. *, **, ***, **** means p < 0.05, 0.01, 0.001, 0.0001. ☆ represents the stroke cavity.