FIG. 3.

Phosphorylation of Erk1/2, p38, and JNK/SAPK MAP kinases in LPS-stimulated CD14-CHO cells and its inhibition by anti-CD14 antibody. (A) Phosphorylation of the MAP kinases in LPS-stimulated CD14-CHO cells. CD14-CHO cells and mock-transfected control CHO cells were cultured with LPS (0.1 μg/ml) for 30 min. (B) Inhibition LPS-induced phosphorylation of the MAP kinases by anti-CD14 antibody. CD14-CHO cells were pretreated with anti-CD14 antibody (5 μg/ml) for 4 h and then treated with LPS (0.1 μg/ml) for 30 min. The cells were lysed, and the lysates were analyzed by immunoblotting using an antibody to Erk1/2, phospho-Erk1/2, p38, phospho-p38, JNK/SAPK, or phospho -JNK/SAPK.