FIG. 4.

Augmentation of LPS-induced DNA synthesis by a p38 inhibitor, SB203580. CD14-CHO cells or mock-transfected control CHO cells were pretreated with SB203580 (20 μM), PD98059 (20 μM), or NPPB (10 μM) (A) or with various concentrations of SB203580 (B) for 1 h and then treated with LPS (0.1 μg/ml). DNA synthesis was determined by [³H]thymidine incorporation. (C) Inhibitory action of SB203580 or PD98059 on the phosphorylation of p38 and Erk1/2 was confirmed by immunoblotting using an antibody to p38, phospho-p38, Erk1/2, or phospho-Erk1/2.