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. 2022 Dec 27;9(6):064702. doi: 10.1063/4.0000171

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FIG. 6. — (a) SEC –MALS–SAXS chromatogram displaying the integrated intensity of diffraction and radius of gyration (R_g) resulting from each frame of data collected for TraG*_R100 (blue) and _ΔTraG*_R100 (red). (b) Scattering profiles of TraG*_R100 (blue) and _ΔTraG*_R100 (red) show the abundance of noise in the TraG*_R100 profile and noise in the high q regions for _ΔTraG*_R100. (c) P(r) functions of TraG*_R100 (blue) and _ΔTraG*_R100 (red) were produced using GNOM(IFT) and optimized for R_g values comparable to those resulting from Guinier analysis; Dmax of TraG* was truncated at 450 Å while _ΔTraG* was cut at 175 Å to allow for some. (d) Normalized Kratky plots resulting from the scattering profile of TraG*_R100 (blue) and _ΔTraG*_R100 (red). (e) A bead model of _ΔTraG*_R100 which displays the smallest shell (red) with mesh models (green) of the highest shell from DAMCLUST refinement, produced from the P(r) function. This reconstruction had an ambiguity score of 1.940 with 87 ambiguity categories based on AMBIMETER. All SAXS data images were produced using RAW, and bead models were visualized using Pymol.^52,103