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. 2022 Dec 21;2022:5297603. doi: 10.1155/2022/5297603

Table 5.

Summary of stability of four analytes in plasma and tissue samples (n = 6).

Compounds Matrix Concentration (ng/mL) Ambient temperature for 4 h Autosampler at 4°C for 8 h Three freeze-thraw cycles −80°C for 30 days
Accuracy RSD (%) Accuracy RSD (%) Accuracy RSD (%) Accuracy RSD (%)
Chlorogenic acid Plasma 2 101.68 ± 8.39 8.25 98.25 ± 7.83 7.97 101.23 ± 12.44 12.29 93.35 ± 10.78 11.55
4 86.78 ± 6.16 7.09 97.90 ± 11.88 12.14 95.02 ± 5.39 5.67 98.92 ± 12.32 12.46
100 112.33 ± 3.72 3.31 111.33 ± 4.37 3.92 100.68 ± 6.22 6.18 113.17 ± 4.96 4.38
2400 98.40 ± 5.56 5.65 99.47 ± 7.59 7.63 91.98 ± 4.75 5.17 112.67 ± 7.58 6.73
Heart 2 102.73 ± 12.98 12.64 101.70 ± 10.91 10.72 91.72 ± 6.33 6.90 88.98 ± 5.73 6.43
4 96.88 ± 11.06 11.42 99.50 ± 6.21 6.24 99.03 ± 10.17 10.27 102.27 ± 10.30 10.07
100 112.83 ± 9.11 8.07 111.93 ± 11.77 10.52 111.00 ± 7.72 6.96 112.62 ± 8.15 7.24
2400 108.60 ± 8.41 7.75 101.43 ± 10.34 10.20 110.55 ± 8.11 7.33 104.85 ± 10.69 10.19
Liver 2 88.47 ± 6.93 7.83 87.82 ± 6.45 7.35 88.47 ± 7.22 8.16 87.43 ± 7.74 8.86
4 86.27 ± 7.32 8.48 87.33 ± 5.74 6.57 86.00 ± 6.23 7.24 87.30 ± 4.35 4.98
100 99.82 ± 2.89 2.89 106.33 ± 2.88 2.70 102.27 ± 4.97 4.86 87.13 ± 5.77 6.62
2400 104.17 ± 2.14 2.05 100.92 ± 3.08 3.05 99.98 ± 1.86 1.86 87.02 ± 5.24 6.03
Spleen 2 97.62 ± 8.11 8.30 109.83 ± 9.95 9.06 105.55 ± 7.56 7.17 109.70 ± 13.05 11.90
4 105.82 ± 9.91 9.37 100.15 ± 9.69 9.67 103.68 ± 9.71 9.36 98.88 ± 4.16 4.20
100 112.00 ± 7.82 6.98 111.12 ± 7.31 6.58 106.82 ± 6.88 6.44 111.17 ± 7.17 6.45
2400 101.52 ± 7.73 7.61 104.00 ± 7.45 7.17 106.67 ± 5.43 5.09 104.73 ± 7.04 6.72
Lung 2 103.27 ± 8.01 7.75 111.67 ± 5.43 4.86 112.93 ± 9.57 8.48 113.77 ± 9.20 8.08
4 101.83 ± 6.41 6.30 100.55 ± 8.58 8.53 100.07 ± 10.39 10.38 98.77 ± 6.15 6.23
100 111.50 ± 5.47 4.90 110.17 ± 10.93 9.92 110.17 ± 7.14 6.48 110.03 ± 9.33 8.46
2400 100.67 ± 10.17 10.11 98.78 ± 7.01 7.10 104.88 ± 8.23 7.85 110.17 ± 4.54 4.12
Kidney 2 110.07 ± 7.34 6.67 88.18 ± 4.98 5.64 97.22 ± 7.67 7.89 89.87 ± 9.23 10.27
4 87.63 ± 7.76 8.86 88.40 ± 6.19 7.00 87.60 ± 9.26 10.58 89.60 ± 9.05 10.10
100 112.00 ± 7.01 6.26 111.92 ± 8.86 7.91 111.17 ± 7.44 6.69 103.22 ± 11.32 10.97
2400 100.55 ± 5.11 5.09 98.10 ± 4.87 4.97 100.45 ± 3.96 3.94 101.23 ± 4.69 4.63
Small intestine 2 105.00 ± 8.20 7.81 102.63 ± 3.52 3.43 103.90 ± 4.49 4.32 101.42 ± 7.82 7.71
4 91.73 ± 4.49 4.89 99.47 ± 5.10 5.13 99.78 ± 7.70 7.72 106.70 ± 5.43 5.09
100 95.90 ± 12.31 12.84 108.67 ± 12.04 11.08 100.93 ± 12.33 12.22 99.07 ± 12.41 12.52
2400 112.00 ± 5.37 4.79 111.67 ± 7.26 6.50 111.67 ± 6.12 5.48 110.83 ± 6.68 6.02
Stomach 2 113.08 ± 11.06 9.78 111.67 ± 6.25 5.60 113.58 ± 8.22 7.24 109.08 ± 9.97 9.14
4 102.70 ± 10.17 9.90 97.82 ± 9.05 9.25 98.87 ± 9.82 9.94 89.87 ± 7.04 7.84
100 93.75 ± 8.65 9.22 94.82 ± 5.05 5.33 93.47 ± 6.76 7.23 107.33 ± 5.89 5.49
2400 107.17 ± 8.68 8.10 96.60 ± 3.60 3.72 100.23 ± 4.63 4.62 99.73 ± 10.09 10.12
Brain 2 110.33 ± 7.55 6.85 106.15 ± 12.36 11.65 105.65 ± 10.92 10.34 90.73 ± 10.25 11.30
4 89.12 ± 9.00 10.10 87.57 ± 8.47 9.67 90.68 ± 10.51 11.59 105.32 ± 9.72 9.23
100 91.93 ± 9.93 10.80 109.17 ± 7.44 6.82 108.13 ± 9.91 9.17 105.48 ± 6.92 6.56
2400 102.93 ± 5.79 5.62 106.72 ± 9.83 9.21 96.57 ± 10.12 10.48 97.98 ± 9.56 9.75
Naucleactonin C Plasma 5 101.95 ± 10.33 10.14 106.67 ± 10.88 10.20 91.87 ± 8.63 9.39 109.57 ± 12.66 11.55
8 108.88 ± 1.87 1.72 105.01 ± 6.32 6.02 105.88 ± 1.87 1.77 113.33 ± 5.32 4.69
140 87.67 ± 9.20 10.50 87.88 ± 4.14 4.71 111.88 ± 1.87 1.67 111.57 ± 9.60 8.60
2400 88.93 ± 6.01 6.75 89.25 ± 7.58 8.49 89.17 ± 7.49 8.40 113.83 ± 7.14 6.27
Heart 5 111.97 ± 11.63 10.39 100.78 ± 9.63 9.56 107.98 ± 9.45 8.75 107.42 ± 10.24 9.53
8 110.50 ± 4.42 4.00 107.25 ± 9.25 8.62 109.08 ± 8.25 7.56 110.87 ± 9.04 8.15
140 102.78 ± 6.89 6.71 104.88 ± 3.80 3.62 100.40 ± 9.13 9.09 98.28 ± 5.70 5.80
2400 112.33 ± 10.41 9.26 111.50 ± 5.68 5.10 112.33 ± 5.72 5.09 110.50 ± 4.81 4.35
Liver 5 101.78 ± 11.83 11.63 107.70 ± 5.36 4.98 98.32 ± 6.66 6.78 98.38 ± 12.07 12.27
8 87.60 ± 5.90 6.73 86.47 ± 4.52 5.23 90.60 ± 4.91 5.42 86.00 ± 8.60 10.00
140 88.57 ± 2.44 2.76 95.00 ± 4.15 4.37 96.28 ± 4.36 4.53 97.32 ± 7.17 7.36
2400 98.93 ± 3.81 3.85 91.37 ± 2.51 2.75 89.35 ± 3.70 4.15 89.97 ± 7.32 8.14
Spleen 5 110.33 ± 8.71 7.89 112.92 ± 8.72 7.72 110.67 ± 8.57 7.75 109.68 ± 12.57 11.46
8 99.28 ± 13.31 13.41 93.42 ± 8.00 8.57 96.13 ± 11.98 12.46 101.90 ± 10.25 10.06
140 93.13 ± 6.10 6.55 95.27 ± 7.08 7.43 89.18 ± 8.23 9.23 92.35 ± 2.70 2.92
2400 103.33 ± 8.37 8.10 105.67 ± 8.50 8.05 107.73 ± 8.10 7.51 109.83 ± 4.96 4.51
Lung 5 109.50 ± 11.47 10.48 100.40 ± 10.26 10.22 101.05 ± 5.33 5.28 95.98 ± 10.47 10.91
8 94.73 ± 8.92 9.41 91.40 ± 9.71 10.63 101.90 ± 10.45 10.25 99.03 ± 10.49 10.59
140 102.38 ± 4.12 4.03 94.45 ± 5.92 6.27 93.75 ± 8.53 9.09 92.58 ± 3.60 3.89
2400 111.37 ± 9.27 8.33 111.85 ± 9.53 8.52 112.125 ± 9.66 8.61 111.67 ± 3.39 3.03
Kidney 5 109.33 ± 7.55 6.91 106.08 ± 8.96 8.44 98.92 ± 9.91 10.02 97.18 ± 10.46 10.76
8 99.10 ± 4.24 4.27 97.38 ± 7.31 7.51 91.10 ± 9.20 10.10 94.77 ± 7.62 8.04
140 96.08 ± 7.36 7.66 95.43 ± 7.99 8.37 101.10 ± 4.98 4.92 100.25 ± 8.25 8.23
2400 108.00 ± 5.62 5.20 103.87 ± 4.68 4.51 104.57 ± 6.09 5.82 102.30 ± 5.90 5.77
Small intestine 5 111.90 ± 12.07 10.79 111.67 ± 8.14 7.29 111.17 ± 8.33 7.49 112.67 ± 8.78 7.79
8 103.33 ± 3.39 3.28 100.03 ± 11.46 11.46 100.60 ± 10.24 10.18 104.25 ± 9.49 9.10
140 88.60 ± 9.48 10.70 89.80 ± 8.30 9.24 87.88 ± 5.57 6.34 90.17 ± 7.26 8.05
2400 107.48 ± 8.23 7.66 103.75 ± 6.68 6.44 108.43 ± 8.65 7.98 102.32 ± 8.65 8.45
Stomach 5 111.00 ± 7.90 7.12 112.17 ± 7.25 6.46 113.12 ± 9.97 8.81 113.83 ± 2.99 2.63
8 107.32 ± 12.96 12.08 98.37 ± 10.75 10.93 105.90 ± 9.13 8.62 103.55 ± 9.49 9.16
140 88.15 ± 7.91 8.98 86.38 ± 8.38 9.70 87.12 ± 5.24 6.1 88.28 ± 6.31 7.15
2400 100.53 ± 7.29 7.25 99.52 ± 5.85 5.88 104.90 ± 5.53 5.28 102.32 ± 8.22 8.03
Brain 5 106.35 ± 11.82 11.11 109.83 ± 6.05 5.51 98.92 ± 10.65 10.77 104.10 ± 10.88 10.45
8 88.87 ± 10.43 11.74 90.65 ± 6.37 7.03 89.95 ± 9.23 10.26 88.82 ± 10.57 11.90
140 88.33 ± 6.74 7.63 87.92 ± 8.15 9.27 87.20 ± 4.98 5.71 87.93 ± 5.81 6.61
2400 99.43 ± 6.38 6.41 112.67 ± 6.62 5.88 109.67 ± 6.44 5.87 109.00 ± 5.22 4.78
Khaephuoside A Plasma 5 99.10 ± 9.25 9.33 90.97 ± 9.01 9.90 93.93 ± 11.26 11.98 101.18 ± 3.53 3.49
8 91.38 ± 12.76 13.96 94.88 ± 12.31 12.97 90.67 ± 9.29 10.25 108.17 ± 9.20 8.50
120 101.35 ± 4.33 4.28 101.32 ± 3.40 3.36 96.75 ± 4.55 4.70 107.30 ± 11.48 10.70
1800 99.35 ± 6.04 6.08 101.93 ± 6.90 6.77 96.13 ± 4.03 4.19 112.67 ± 7.66 6.80
Heart 5 107.60 ± 11.23 10.44 104.15 ± 9.92 9.52 110.67 ± 10.82 9.78 107.45 ± 9.35 8.70
8 112.67 ± 7.23 6.42 111.83 ± 8.23 7.36 111.33 ± 5.92 5.32 101.00 ± 12.05 11.93
120 111.67 ± 6.22 5.57 112.67 ± 5.89 5.23 108.58 ± 11.51 10.60 113.50 ± 7.20 6.35
1800 110.83 ± 2.79 2.51 106.58 ± 8.77 8.22 113.67 ± 4.27 3.76 101.90 ± 12.30 12.07
Liver 5 93.87 ± 9.31 9.92 106.17 ± 4.88 4.59 95.62 ± 9.82 10.27 100.87 ± 10.27 10.19
8 108.95 ± 10.77 9.88 101.97 ± 7.93 7.78 105.20 ± 10.21 9.70 91.12 ± 10.79 11.84
120 107.67 ± 6.22 5.78 111.50 ± 7.26 6.51 101.62 ± 9.62 9.47 104.23 ± 8.68 8.33
1800 108.83 ± 2.32 2.13 109.83 ± 5.38 4.90 103.20 ± 7.52 7.29 108.93 ± 9.52 8.74
Spleen 5 110.30 ± 12.31 11.16 112.17 ± 7.94 7.07 111.00 ± 10.26 9.24 111.50 ± 8.41 7.54
8 112.17 ± 5.64 5.02 111.67 ± 6.19 5.54 111.38 ± 7.36 6.58 110.67 ± 7.17 6.48
120 107.85 ± 7.83 7.26 105.45 ± 7.95 7.54 98.83 ± 7.51 7.60 105.42 ± 10.79 10.24
1800 109.77 ± 8.61 7.85 111.17 ± 8.18 7.36 113.17 ± 3.31 2.93 111.83 ± 7.57 6.77
Lung 5 113.33 ± 8.71 7.69 106.65 ± 12.43 11.65 98.87 ± 9.02 9.12 102.98 ± 9.50 9.23
8 105.32 ± 7.81 7.41 103.18 ± 10.97 10.63 105.03 ± 9.86 9.38 104.00 ± 11.73 11.28
120 101.27 ± 3.53 3.49 99.07 ± 11.01 11.11 102.02 ± 6.36 6.23 110.83 ± 5.19 4.69
1800 110.83 ± 8.23 7.43 112.80 ± 7.46 6.61 110.00 ± 8.88 8.07 112.50 ± 6.80 6.05
Kidney 5 96.42 ± 6.24 6.47 99.65 ± 10.53 10.57 101.62 ± 11.37 11.19 112.78 ± 11.43 10.13
8 113.17 ± 6.46 5.71 110.83 ± 7.88 7.11 113.00 ± 6.99 6.18 113.17 ± 7.19 6.36
120 111.67 ± 5.32 4.76 111.83 ± 5.31 4.75 112.00 ± 5.93 5.30 112.00 ± 5.62 5.02
1800 102.03 ± 4.44 4.35 97.37 ± 6.84 7.02 100.50 ± 5.53 5.50 101.12 ± 6.56 6.48
Small intestine 5 110.02 ± 8.19 7.44 112.83 ± 3.43 3.04 100.88 ± 9.39 9.30 94.98 ± 9.42 9.91
8 105.62 ± 9.76 9.24 109.53 ± 10.14 9.25 101.00 ± 10.42 10.31 107.70 ± 11.47 10.65
120 103.30 ± 7.75 7.50 106.00 ± 7.29 6.88 102.80 ± 6.09 5.93 101.58 ± 6.26 6.16
1800 113.00 ± 6.13 5.43 110.83 ± 8.13 7.34 113.67 ± 8.19 7.20 113.12 ± 12.47 11.02
Stomach 5 113.68 ± 8.42 7.41 112.13 ± 10.23 9.12 111.12 ± 10.43 9.38 110.67 ± 6.09 5.50
8 111.67 ± 6.59 5.90 111.00 ± 5.22 4.70 109.67 ± 3.33 3.03 104.50 ± 4.71 4.51
120 113.17 ± 3.66 3.23 111.50 ± 5.86 5.25 112.17 ± 6.15 5.48 110.92 ± 8.36 7.53
1800 109.83 ± 7.36 6.70 111.92 ± 10.99 9.82 110.50 ± 6.28 5.69 113.83 ± 5.56 4.89
Brain 5 109.82 ± 10.26 9.34 106.90 ± 12.09 11.31 103.05 ± 9.12 8.85 112.50 ± 5.58 4.96
8 111.00 ± 7.42 6.52 112.50 ± 7.06 6.28 113.33 ± 3.83 3.38 111.33 ± 6.09 5.47
120 112.50 ± 5.13 4.56 112.33 ± 6.47 5.76 113.17 ± 5.31 4.69 110.17 ± 8.28 7.52
1800 105.82 ± 8.31 7.86 112.83 ± 7.68 6.81 110.83 ± 7.52 6.79 113.83 ± 4.49 3.95
3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside Plasma 25 86.25 ± 5.40 6.26 94.95 ± 9.80 10.32 102.87 ± 10.37 10.08 105.60 ± 10.19 9.65
50 87.20 ± 7.91 9.07 98.98 ± 11.30 11.30 94.53 ± 9.84 10.41 107.88 ± 14.96 13.87
350 112.83 ± 4.58 4.06 112.67 ± 6.62 5.88 108.33 ± 5.13 4.73 109.07 ± 1.87 1.72
2400 89.98 ± 4.76 5.29 89.43 ± 7.42 8.30 86.07 ± 3.39 3.93 111.74 ± 3.76 3.37
Heart 25 98.10 ± 9.43 9.61 100.45 ± 10.89 10.85 97.55 ± 9.01 9.24 88.38 ± 5.69 6.44
50 94.60 ± 11.14 7.80 102.70 ± 7.98 7.77 97.05 ± 8.42 8.68 99.12 ± 9.56 9.65
350 102.57 ± 4.92 4.80 112.63 ± 98.93 7.93 110.33 ± 5.82 5.27 108.38 ± 7.34 6.77
2400 102.23 ± 6.64 6.49 92.98 ± 8.87 9.54 101.57 ± 6.73 6.63 99.57 ± 9.46 9.50
Liver 25 91.10 ± 10.37 11.39 102.88 ± 10.85 10.54 97.32 ± 10.44 10.73 104.70 ± 11.97 11.43
50 105.60 ± 9.04 8.56 112.50 ± 5.65 5.02 107.48 ± 10.54 9.80 95.25 ± 11.29 11.86
350 97.50 ± 6.63 6.80 113.00 ± 4.00 3.54 103.12 ± 5.26 5.10 108.67 ± 8.98 8.27
2400 104.32 ± 10.18 9.76 100.42 ± 6.85 6.83 96.25 ± 5.74 5.96 107.68 ± 7.73 7.18
Spleen 25 88.72 ± 9.93 11.19 104.47 ± 9.00 8.62 90.93 ± 9.15 10.07 112.83 ± 3.60 3.19
50 113.17 ± 10.59 9.36 108.35 ± 10.58 9.77 95.62 ± 10.90 11.40 107.68 ± 10.39 9.65
350 111.33 ± 7.63 6.86 111.68 ± 9.21 8.25 112.17 ± 7.99 7.12 112.17 ± 8.40 7.49
2400 102.27 ± 11.50 11.24 112.00 ± 8.67 7.74 105.32 ± 6.59 6.26 111.00 ± 6.03 5.44
Lung 25 90.52 ± 10.77 11.90 96.80 ± 11.29 11.67 97.07 ± 10.87 11.20 97.92 ± 11.34 11.59
50 112.67 ± 7.84 6.96 111.02 ± 7.81 7.04 100.02 ± 12.46 12.46 109.25 ± 11.46 10.49
350 111.33 ± 6.89 6.19 109.47 ± 11.10 10.14 103.68 ± 11.62 11.21 112.33 ± 6.92 6.16
2400 98.85 ± 9.83 9.94 101.82 ± 8.67 8.52 100.62 ± 7.71 7.66 109.12 ± 10.69 9.79
Kidney 25 100.05 ± 11.92 11.92 101.42 ± 8.63 8.51 96.23 ± 9.14 9.50 110.83 ± 6.24 5.63
50 98.37 ± 7.53 7.65 102.72 ± 10.29 10.02 106.75 ± 9.15 8.57 99.88 ± 9.65 9.66
350 90.05 ± 8.47 9.40 102.03 ± 4.61 4.52 111.83 ± 7.03 6.28 105.40 ± 10.28 9.75
2400 104.72 ± 8.96 8.56 106.27 ± 10.19 9.59 107.85 ± 9.16 8.49 104.82 ± 8.59 8.20
Small intestine 25 87.47 ± 7.17 8.20 96.42 ± 9.13 9.47 94.10 ± 8.05 8.55 99.25 ± 6.06 6.11
50 109.83 ± 2.56 2.33 100.75 ± 6.11 6.07 106.83 ± 5.34 5.00 101.80 ± 8.78 8.62
350 111.17 ± 7.73 6.95 113.67 ± 8.76 7.70 113.00 ± 3.79 3.36 106.33 ± 5.39 5.07
2400 98.50 ± 5.49 5.57 104.65 ± 7.85 7.50 99.92 ± 5.94 5.94 94.90 ± 8.62 9.08
Stomach 25 111.83 ± 5.78 5.17 110.50 ± 4.42 4.00 113.00 ± 6.32 5.60 106.32 ± 9.48 8.92
50 112.75 ± 10.97 9.73 112.67 ± 3.67 3.26 112.83 ± 5.60 4.96 109.83 ± 8.66 7.88
350 113.17 ± 3.87 3.42 108.17 ± 9.68 8.95 111.00 ± 6.20 5.58 111.83 ± 5.98 5.35
2400 101.27 ± 7.89 7.80 94.40 ± 6.00 6.35 96.82 ± 8.08 8.34 103.08 ± 9.60 9.32
Brain 25 99.82 ± 9.82 9.84 107.80 ± 12.79 11.86 100.68 ± 9.80 9.73 105.37 ± 7.31 6.93
50 109.35 ± 12.15 11.11 112.83 ± 4.26 3.78 113.77 ± 10.36 9.11 113.83 ± 3.87 3.40
350 111.50 ± 8.64 7.75 110.83 ± 7.47 6.74 111.17 ± 7.63 6.27 112.83 ± 7.78 6.90
2400 98.95 ± 3.97 4.01 100.10 ± 9.43 9.42 98.23 ± 5.39 5.49 101.50 ± 9.94 9.80