| Reagent | Final concentration | Amount |
|---|---|---|
| Calcium chloride (CaCL 2 ) | 1 mM | 500 µL |
| Magnesium sulfate (MgSO 4 ) | 1 mM | 500 µL |
| PBS | n/a | 499 mL |
| Total | n/a | 500 mL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.