| Component | Volume (×1) | Volume (×96) |
| T4 DNA ligase reaction buffer | 0.4 µL | 38.4 µL |
| T4 PNK | 0.2 µL | 19.2 µL |
| ddH 2 O | 1.4 µL | 134.4 µL |
| Master mix | 2 µL | 192 µL |
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