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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2022 Nov 17;638:127–133. doi: 10.1016/j.bbrc.2022.11.047

Figure 1. Gel bands for mass spectrometry analysis.

Figure 1.

(A) The western blot image using anti-HP antibody for mass spectrometry. The gel was run to focus on bands with a molecular weight (MW) greater than 30 kDa since the target protein, zonulin as pre-HP2, has a MW ~50 kDa. Lane 1: HP1-1 human serum, Lane 2: HP2-1 human serum, Lane 3: HP2-2 human serum. (B) Image of the gel sent for mass spectrometry analysis with the excised bands. HP:haptoglobin