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. 2022 Dec 15;16:1010121. doi: 10.3389/fncel.2022.1010121

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Copyright © 2022 Kolpakova, van der Vinne, Gimenez-Gomez, Le and Martin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optogenetic stimulation of ChIs decreases sEPSCs frequency in D1-MSNs. (A) Schematic of the experimental setup: whole cell recording of D1-MSNs sEPSC during optogenetic stimulation of ChIs. (B) Same slice images of representative MSNs [(i), DIC], corresponding IV traces to confirm MSN identity (ii), red-epifluorescence to verify the cell is D1R+ (iii), and eYFP epifluorescence of ChI cell in proximity of the recording (iv). Scale bar 10 um. (Ci) Representative sEPSCs in D1-MSNs in ChAT-ChR2 mice before (Pre) and after (Post) ChI optogenetic stimulation (blue arrowheads). (Cii) Average sEPSCs inter-event intervals (IEI) in Pre (white circles) and Post (blue circles) ChI optogenetic stimulation in ChAT-ChR2 D1-MSNs (n = 14). (Di) Representative traces of sEPSCs in D1-MSNs of tdTomato control mice before (Pre) and after (Post) ChI stimulation. (Dii) Average EPSCs inter-event intervals (IEI) in Pre (white circles) and Post (gray circles) ChI optogenetic stimulation in control tdTomato D1-MSNs (n = 10). (E) Cumulative frequency distribution of D1-MSN sEPSCs IEI in ChAT-ChR2 mice group Pre (black traces) and Post (blue traces) ChI optogenetic stimulation. Each solid line represents a neuron. Average traces for Pre and Post conditions are shown in dotted black and blues lines, respectively. Inset. Cumulative distributions of ChAT-ChR2 D1-MSNs EPSCs IEIs broken into percentiles of distribution to quantify median (50%), shoulders (25 and 75%), and extreme values (5 and 94%) of distribution, that are 1¹⁰ transformed to normalize the distribution. (F) Cumulative frequency distribution of D1-MSNs inter-event intervals (IEI) of sEPSCs before (Pre, black lines) and after (Post, gray lines) ChI optogenetic stimulation in tdTomato control mice. Each solid line represents a neuron. Average traces are shown in dotted black and blues lines for Pre and Post conditions, respectively. Inset. Same as inset in panel (E), but in tdTomato D1-MSN controls. (G) Percentiles of cumulative distribution of transformed IEIs of EPSCs in ChAT-ChR2 D1-MSNs (Pre, white circles, Post, blue circles) and control TdTomato D1-MSNs (Pre, white circles, Post, gray circles). *p < 0.05, ns, no significant difference.