Figure 1.

Urolithin B is the (poly)phenol metabolite with better potential for hampering IAPP aggregation (A) Docking simulations of IAPP and (poly)phenols by AutoDock Vina reveals urolithins as the best performing molecules. (B) Vacuum electrostatics of urolithin A (UroA) and urolithin B (UroB) docking in IAPP using Pymol (upper panel). Predicted bond analysis of UroA and UroB with IAPP amino acid residues (lower panel). Green – π-stacking. Pink – Hydrophobic interactions. Blue – hydrogen bonds. Yellow – Salt bridges. (C) The frequency of propidium iodide (PI) positive cells was assessed by flow cytometry in cells expressing ppIAPP and the respective control in the presence of UroA (left) and UroB (right). The values represent mean ± SD from at least three independent experiments. Statistical differences are denoted as ^*** p < 0.001 vs. the control condition. E – cells containing the empty vector. I – cells expressing ppIAPP.