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Survival of A. castellanii and growth of intracellular L. pneumophila at 37°C. The y axis on the left represents the number of viable amoeba as a function of time in PYG broth determined by the Celltiter 96 AQueous assay. The y axis on the right represents the number of L. pneumophila CFU ml−1 as a function of time. +Lp, with L. pneumophila; −Lp, without L. pneumophila. Error bars, standard deviations.
