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. 2022 Dec 15;13:1049178. doi: 10.3389/fmicb.2022.1049178

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Copyright © 2022 Kafi, Emaneini, Halimi, Rahdar, Jabalameli and Beigverdi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Derivative melting curves of HRM analysis of five different species generated from urine samples, each displaying a unique profile with respect to shape and melting temperature. Each line represents one sample and the same colors illustrate the same pathogens. Yellow curve (S. saprophyticus, n = 2); light orange (GBS, n = 1); plum (E. faecalis, n = 2); blue (E. coli, n = 5) and dark yellow (K. pneumoniae, n = 4). Pre-melt region: The set of lines to the left of the peak displays the pre-melt start and stop temperatures when every amplicon is double-stranded. Post-melt region: The set of lines to the right of the peak displays the post-melt start and stop temperatures when every amplicon is single-stranded. The HRM analysis was performed with a pre-melt region 73.2–73.6°C and a post-melt region 93.3–93.7°C. No amplification occurred in DNA samples of urogenital flora and therefore no melting curve was observed.