Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Relevant characteristic | Source |
|---|---|---|
| G. parasuis strains | ||
| CF7066 | Serovar 5, wild type strain | Laboratory collection |
| CF7066ΔespP1 | espP1 gene is replaced with an erythromycin resistance cassette | Laboratory collection |
| CF7066ΔespP2 | espP2 gene is replaced with a kanamycin resistance cassette | Laboratory collection |
| CF7066ΔespP1ΔespP2 | espP1 and espP2 are deleted simultaneously and replaced with erythromycin and kanamycin resistance cassette | Laboratory collection |
| E. coli strains | ||
| DH5α | Standard cloning vector | Invitrogen, Carlsbad, CA, USA |
| BL21 (DE3) | Standard expression vector | Invitrogen, Carlsbad, CA, USA |
| Plasmids | ||
| pET-28a | An expression vector, containing N/C-terminal His-tag, Kanr | Novagen, Madison, WI, USA |
| pET-28a-espP1 | pET-28a containing espP1 wild type | This study |
| pET-28a-espP1C47A | pET-28a containing espP1 C47A | This study |
| pET-28a-espP1D144A | pET-28a containing espP1 D144A | This study |
| pET-28a-espP1H172A | pET-28a containing espP1 H172A | This study |
| pET-28a-espP1D195A | pET-28a containing espP1 D195A | This study |
| pET-28a-espP2 | pET-28a containing espP2 wild type | This study |
| pET-28a-espP2C47A | pET-28a containing espP2 C47A | This study |
| pET-28a-espP2D144A | pET-28a containing espP2 D144A | This study |
| pET-28a-espP2H172A | pET-28a containing espP2 H172A | This study |
| pET-28a-espP2D194A | pET-28a containing espP2 D194A | This study |
Kanr, kanamycin-resistance.