. 2022 Dec 15;13:1041774. doi: 10.3389/fmicb.2022.1041774

Table 2.

Primers used in this study.

Primers	Sequences (5′–3′)	Size (bp)
espP1-F/R	GCGGGATCCGACGATGTCTACTGGG	2,766
espP1-F/R	CCGCTCGAGGAACGAGTATCTTACATTGG	2,766
espP1^C47A-F/R	TATCCTAACCAAGCCTGGGGAGCTGTTGCAGGA	8,101
espP1^C47A-F/R	CCAGGCTTGGTTAGGATATTGAAAATCAGCAGT	8,101
espP1^D144A-F/R	GCGTTACGCCTCTAATGCAGCTTTAGTTACAAAATCAT	8,100
espP1^D144A-F/R	CATTAGAGGCGTAACGCTCAGTCCAAAATGGAC	8,100
espP1^H172A-F/R	ATGGACAGCCACTGTGACTTTATGGGGCATTGA	8,100
espP1^H172A-F/R	TCACAGTGGCTGTCCATGATGTTAGCGCGGCA	8,100
espP1^D195A-F/R	TATCAGTGCCTCTGTAGCAGATCAGGCAGGAA	8,100
espP1^D195A-F/R	CTACAGAGGCACTGATATAACCTTTTTTAATTTTGCC	8,100
espP2-F/R	CGCGGATCCCAGACTTATTGGGCAAG	2,289
espP2-F/R	CCGCTCGAGGAACGAGTATCTTACATTGG	2,289
espP2^C47A-F/R	ATCCAAATCAGGCCTGGGGTGCTGTTGCAGGA	7,617
espP2^C47A-F/R	CCAGGCCTGATTTGGATATTGTAAATCAGCAGT	7,617
espP2^D144A-F/R	ACGTTATGCCTCGGATGCCAAATTAGTCACTAAA	7,623
espP2^D144A-F/R	CATCCGAGGCATAACGTTCCGTCCAGAATGGA	7,623
espP2^H172A-F/R	ACGCCACCGTAACCTTATGGGGAATTGAAGTT	7,618
espP2^H172A-F/R	TAAGGTTACGGTGGCGTGCTGAGAAGTGAGTGCTGCA	7,618
espP2^D194A-F/R	GATGGATTAGTGCCTCTGTTAAGGATAAAGCTGGAAATCT	7,618
espP2^D194A-F/R	CAGAGGCACTAATCCATCCTTTTTTTATCTTACC	7,618
espP autotransporter domain-F/R	CGCGGATCCATATGGGCTAGAGTATTAGG	771
espP autotransporter domain-F/R	CCGCTCGAGGAACGAGTATCTTACATTGG	771

Restriction enzyme sites are underlined.