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. 2022 Dec 15;10:1031872. doi: 10.3389/fcell.2022.1031872

FIGURE 5.

FIGURE 5

Pharmacological inhibition of the NAc shell alleviates TLE seizures. (A) Schematic diagram of muscimol microinjection to inhibit the NAc shell and 10 min later KA microinjection into BLA to induce TLE seizures of the C57BL/6 mouse. (B) Racine’s scaling of the mice treated with DMSO control (n = 11 mice) or muscimol (n = 11 mice), showing the maximal seizure stage every 10 min during the whole course of 120 min after KA microinjection. ***p < 0.001, two-way ANOVA with repeated-measures. (C) The onset time of the first seizure after KA microinjection. DMSO control: n = 11 mice; muscimol n = 11 mice; Student’s unpaired t-test. (D) Latency to secondarily general seizures (GSs) after KA microinjection. DMSO control: n = 11 mice; muscimol n = 11 mice; Student’s unpaired t-test. (E) Total number of secondarily GSs after KA microinjection. DMSO control: n = 11 mice; muscimol n = 11 mice; ***p < 0.001, Student’s unpaired t-test. (F) Maximal seizure stage in the whole course of 120 min after KA microinjection. DMSO control: n = 11 mice; muscimol n = 11 mice; **p < 0.01, Mann–Whitney rank sum Test. (G) Mortality rate after KA microinjection. DMSO control: n = 11 mice; muscimol n = 11 mice; chi-square test.