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. 2022 Dec 27;222(2):e202203105. doi: 10.1083/jcb.202203105

Figure 5.

Figure 5.

In spv-1(RNAi) conditions, RHGF-1 without its PDZ domain behaves like a complete loss of function. (A and B) Dwell time and successful transits of wild type, rhgf-1(ok880), and rhgf-1(ok880) expressing full-length RHGF-1 or RHGF-1 lacking its PDZ domain (ΔPDZ). N ≥ 8. Error bars in A are ± SEM. Statistical comparisons for A were performed by Brown-Forsythe ANOVA-Dunnett’s T3 multiple comparisons test. Statistical comparisons for B were performed by individuals’ chi-square tests. (C) Representative stitched DIC images of embryos from rhgf-1(ok880) hermaphrodites expressing full-length RHGF-1 or RHGF-1 lacking its PDZ domain (ΔPDZ) treated with control (empty vector) or spv-1(RNAi). Black arrows point to abnormally shaped embryos. Scale bar, 50 µm. (D) Length-to-width ratio of embryos from rhgf-1(ok880) worms expressing full-length RHGF-1 or ΔPDZ treated with control or spv-1(RNAi). N ≥ 19. Box plot from min to max showing the difference in deviation. Statistical comparisons were performed by Brown-Forsythe ANOVA-Dunnett’s T3 multiple comparisons test. (E) Embryonic lethality of the progeny of rhgf-1(ok880) expressing full-length RHGF-1 or ΔPDZ treated with control or spv-1(RNAi). Nrepeats ≥ 4, Nmothers ≥ 120, Nembryos ≥740. Error bars are ± SEM. Statistical comparisons were performed by Brown-Forsythe ANOVA-Dunnett’s T3 multiple comparisons test. (F) Dwell time in rhgf-1(ok880) spermathecae expressing full-length RHGF-1 or ΔPDZ treated with spv-1(RNAi). N = 26. Error bars are mean ± SEM. Statistical comparison was performed by two-tailed unpaired t test. (G) Successful embryos transit through the spermathecae of rhgf-1(ok880) expressing full-length RHGF-1 or ΔPDZ and treated with spv-1(RNAi). N ≥ 40. Statistical comparison was performed by chi-square tests. Stars designate statistical significance (**** P < 0.0001, ** P < 0.01, * P < 0.05, ns P > 0.05). N, number of samples analyzed.