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. 2022 Nov 28;25(1):34. doi: 10.3892/etm.2022.11733

Figure 2.

Figure 2

Silencing of ALOX5 promotes cell proliferation in HG-induced SV40 MES-13 cells. (A and B) SV40 MES-13 cells were transfected with si-ALOX5-1 or si-ALOX5-2 to silence ALOX5, and the protein expression and mRNA level of ALOX5 was assessed using (A) western blotting and (B) reverse transcription-quantitative PCR, respectively. ***P<0.001 vs. si-control. (C) The HG-induced SV40 MES-13 cells were transfected with si-ALOX5 or si-control, and the cell viability was detected using Cell Counting Kit-8 assay. (D) The expression level of Ki67 was detected using immunofluorescence. (E) The expression level of proliferating cell nuclear antigen was detected using immunofluorescence. ***P<0.001 vs. NG; ###P<0.001 vs. HG + si-control. ALOX5, 5-lipoxygenase; HG, high glucose; si-, siRNA; PCNA, proliferating cell nuclear antigen; NG, normal glucose.